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Objective:To analyze the expression of endoplasmic reticulum lipid raft-associated protein 2 (ERLIN2) in glioblastoma tissues, and to analyze its correlation with the clinicopathological features and prognosis of patients with glioblastoma.Methods:The TCGA database was used to analyze the expression level of ERLIN2 mRNA in glioblastoma. KM-plotter was used to analyze the correlation between ERLIN2 expression and prognosis in patients with glioblastoma. The expression level of ERLIN2 protein in 78 cases of glioblastoma tissue was determined by immunohistochemical staining, and then the tissue samples were divided into high expression and low expression groups according to the ERLIN2 expression level. The clinical and pathological data of patients were collected, including age, gender, tumor laterality, recurrence, isocitrate dehydrogenase(IDH) mutation, and the correlation between ERLIN2 protein and clinicopathological characteristics was further analyzed.Results:The mRNA of ERLIN2 was significantly overexpressed in glioblastoma, and the overall survival rate of patients with high ERLIN2 expression was low, suggesting a poor prognosis. The results of immunohistochemistry and clinicopathological analysis showed that ERLIN2 protein was significantly overexpressed in glioblastoma, and its expression level was significantly related to tumor recurrence.Conclusions:ERLIN2 protein is significantly overexpressed in glioblastoma tissues, and its expression level is related to the clinicopathological characteristics and poor prognosis, suggesting that ERLIN2 can be used as a potential prognostic factor for glioblastoma.
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Objective To analyze and discuss the specific effect and mechanism of Guizhi Fuling pill on angiogenesis in rats with endometriosis. Methods A rat model of endometriosis was established in 40 rats in the same batch, they were randomly divided into 4 groups. The model control group did not give any drugs; the low dose of Guizhi Fuling pill group was given 0. 5 g Guizhi Fuling pill every day; the high dose of Guizhi Fuling pill group was given 3. 0 g cinnamon Fuling pill every day; gestation three ketone group was given three ketene 2 g every day. After 4 weeks of continuous administration, the rats were killed and the expression of VEGF and HIF-1 alpha in the rats was determined. The mechanism of Guizhi Fuling pill in the treatment of endometriosis was determined through the expression of VEGF and HIF-1α. Results Guizhi Fuling pill significantly inhibited the level of intravascular VEGF in rat endometriosis and inhibited the level of mRNA expression of HIF-1α. Conclusion Guizhi Fuling pill can inhibit the angiogenesis of endometriosis, and its mechanism is related to the inhibition of VEGF and HIF-1 alpha.
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Through literature method,expert consultation method,K-means algorithm and empirical analysis method,the paper analyzes the methods for evaluation on the layout of first-aid sites,discusses the indexes for scientific evaluation on the rationality of layout of first-aid sites,conducts visualized and practical calculation based on the first-aid related data of Wuhan city in 2015,verifies the rationality and operability of the evaluation indexes,and provides reference for overall allocation of first-aid resources and rational layout of first-aid sites.
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OBJECTIVE To analyze in vitro activity of ertapenem and other antibiotics against clinical bacterial isolates of ESBLs-producing Escherichia coli and Klebsiella pneumoniae.METHODS E.coli and K.pneumoniae were identified by WalkAway40 with 21CN.ESBLs-producing isolates confirmed accordance with the CLSI guidelines.E-test strips were used for determining the minimum inhibitory concentration(MIC) of ertapenem,while disk diffusion test was performed on each isolate using 10 ?g ertapenem disc.RESULTS ESBLs-producing strains were isolated mainly from sputum,urine,secretion and blood,most of them were from nephrology,neurosurgery,Respiratory department and ICU.High resistance rates were shown for both E.coli and K.pneumoniae against cefepime,ciprofloxacin(63.42%),SMZco(61.78%),tobramycin(45.22%)and gentamicin(61.14%).Most K.pneumoniae and E.coli isolates were susceptible to amikacin(78.70%) and piperacillin/tazobactam(81.30%).Tmipenem showed 100.00% of activity the isolates.Ertapenem showed activity against 100.00% ESBLs-producing E.coli,one ESBLs-producing K.pneumoniae isolate showed ertapenem resistance and another one intermediate,the ratio of susceptibility was 99.40%,ertapenem had MIC at which 50.00% and 90.00% of the isolates were inhibited of 0.032 ?g /ml and 1.0 ?g /ml for ESBLs-producing organisms respectively.CONCLUSIONS Ertapenem is less active to ESBLs-producing organisms then imipenem.Ertapenem resistance appears to be the most sensitive indicator for detection of KPCs.
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10pg/ml and IL-8≥70 pg/ml as the critical value,PCT and IL-6 levels had diagnostic sensitivities of 90.6% and 75.0%,and specificities of 83.3% and 77.7%,respectively.The combination of PCT and IL-6 had a sensitivity of 81.2%,and specificity of 94.2%.The positive predictive value was of 96.2% and the negative predictive value of 73.0%.CONCLUSIONS Serum PCT appears to be a promising indicator in differentiating viral infection from bacterial gastroenteritis,but the combination of PCT and IL-6 has a more clinical value.